Effect of interleukin-10 on experimental severe acute pancreatitis's histopathologic score in rats

ObjectiveTo explore effect of interleukin 10 (IL 10) on histopathologic scores of severe acute pancreatitis (SAP) and the level of serum amylase (AMY) in rats.Methods48 female and male adult Sprague Dawley rats (200—300 g) were randomly allocated into three groups: OP group, SAP group and IL 10 group with 16 rats in each group. SAP was made with retrograde ductal infusion of 5% sodium taurocholate solution.ResultsIn SAP and IL 10 group, there were interstitial edema, necrosis, neutrophil infiltration and interstitial hemorrhage of pancreas, more or less. At 6 h and 12 h after models were made, the pancreatic histopathologic score in IL 10 group (4 00±0 33 and 6 25±0 25) were significantly lower than that in SAP group (6 13±0 35 and 9 50± 0 50) ( P 0 01 ). At 6 h after models were made, the serum AMY in IL 10 group was lower than that in SAP group ( P0 05), but at 12 h there were no differences.ConclusionIn earlier period of SAP in rats, IL 10 can lower the serum AMY level, and significantly reduced pancreatic histologic score (edema, inflammation, hemorrhage, and necrosis).