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2007•Journal of Jinan UniversityRequires access

Effect of Trifoliumpratense L.extract on activation and proliferation of mouse T lymphocytes in vitro

Yao Man-lin

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Abstract

Aim:To study the effect of Trifoliumpratense Leguminosae extract(TLE) on activation and proliferation of mouse T lymphocytes in vitro. Methods: The effect of TLE on the side-effect and proliferation of the murine lymphocytes were estimated by MTT test;Double-fluorescent antibody staining plus flow cytometrics were used to detect CD69 expression;CFDA-SE staining plus flow cytometrics to analyze CD3+ T lymphocytes proliferation.Results: TLE can inhibit activation and proliferation of mouse T lymphocytes at the mass concentration of 10、20、30、40 mg/L,and TLE have little side-effect on mouse lymphocytes.Conclusion:TLE can inhibit the activation and proliferation of mouse T lymphocytes in vitro.

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What this paper is about

Aim:To study the effect of Trifoliumpratense Leguminosae extract(TLE) on activation and proliferation of mouse T lymphocytes in vitro. Methods: The effect of TLE on the side-effect and proliferation of the murine lymphocytes were estimated by MTT test;Double-fluorescent antibody staining plus flow cytometrics were used to detect CD69 expression;CFDA-SE staining plus flow cytometrics to analyze CD3+ T lymphocytes proliferation.Results: TLE can inhibit activation and proliferation of mouse T lymphocytes at the mass concentration of 10、20、30、40 mg/L,and TLE have little side-effect on mouse lymphocytes.Conclusion:TLE can inhibit the activation and proliferation of mouse T lymphocytes in vitro.

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Available abstract

Aim:To study the effect of Trifoliumpratense Leguminosae extract(TLE) on activation and proliferation of mouse T lymphocytes in vitro. Methods: The effect of TLE on the side-effect and proliferation of the murine lymphocytes were estimated by MTT test;Double-fluorescent antibody staining plus flow cytometrics were used to detect CD69 expression;CFDA-SE staining plus flow cytometrics to analyze CD3+ T lymphocytes proliferation.Results: TLE can inhibit activation and proliferation of mouse T lymphocytes at the mass concentration of 10、20、30、40 mg/L,and TLE have little side-effect on mouse lymphocytes.Conclusion:TLE can inhibit the activation and proliferation of mouse T lymphocytes in vitro.

Key concepts: In vitro, CD69, Molecular biology, Flow cytometry, Chemistry, CD3, T lymphocyte, Cell growth

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