Effects of puerarin on the proliferation and alkaline phosphatase activity of UMR-106 cells

Objective:To evaluate the effects of puerarin on proliferation and alkaline phosphatase(ALP) activity of UMR106 cell lines.Methods:UMR106 cell lines cultured in α-MEM medium were incubated with puerarin(10~(-8),10~(-7) or 10~(-6)mol·L~(-1)) and 10~(-8) mol·L~(-1) estradiol.The histomorphologic changes of the cells were observed under a microscope.The cellular growth curve was mapped.The ALP activities were assayed using matrix kinetics with p-nitrophenyl-sodium phosphate.Results:Compared with the blank control cell lines,puerarin and 10~(-8) mol·L~(-1) estradiol significantly accelerated the proliferation of the UMR106 cells post the incubation for 24, 48 and 72 h.The proliferation rate of UMR106 cells was 9.3%,23.3%,40.9% and 49.7% post the incubation with 10~(-8),10~(-7),10~(-6) mol·L~(-1) puerarin and 10~(-8) mol·L~(-1) estradiol,respectively,for 48 h.A significant increase of the ALP activity of the UMR106 cells was observed post the incubation with 10~(-6) mol·L~(-1) puerarin for 24 and 48 h compared with the blank control cells(P0.01).An increase of the ALP activity in the MEM media post the incubation with 10~(-6) mol·L~(-1) puerarin for 24 h was found compared with the blank control cells(P0.05).Conclusion:Puerarin showed a positive function on the proliferation of UMR cells and the production of the ALP,suggesting that puerarin plays a role in the proliferation and differentiation of osteoblasts.