RAPD Marker of Restorer Gene for Cytoplasmic Male Sterility in Radish

RAPD markers was evaluated in a F 2 population of 174 plants derived from male fertility restorer line 9802 and male sterile line 9802A.Sterile and restorer DNA pools were established using the sterile and fertile plants among F 2 population, respectively. One hundred primers were used for screening polymorphisms between the two pools, and OPC6 produced distinguishable as well as polymorphic band between the two pools. Linkage analysis in the mapping population showed that the marker, OPC6 1900 , was linked to the restorer gene. The distance between the restorer gene and OPC6 1900 was 11.6 cM. The breeding for new fertility restorer lines of CMS radish would be facilitated by using the marker.