Genetic diversity in kenaf(Hibiscus cannabinus) UG93 cytoplasmic male sterile lines,maintainer lines and restorer lines revealed by ISSR and cpSSR markers

8 ISSR primers and 7 chloroplast SSR(cpSSR)primer pairs were employed to study genetic diversity among 9 pairs of kenaf UG93 cytoplasmic male sterile/maintainer lines and 5 restorer lines.Nucleus genome genetic similarity coefficients(SC) based on ISSR ranged from 0.333 to 1.000,with the average of 0.583,0.689 and 0.812 among maintainer lines,maintainer and restorer lines,and restorer lines respectively.Chloroplast genome SC based on cpSSR ranged from 0 to 0.286.The 23 tested lines were clustered into 4 haplotypes,with all 9 sterile lines were classified into a single type.5 of 9 couple of sterile/maintainer lines showed some degree diversity which indicated that replacement of nucleus genome between sterile line and maintainer line was not complete.The results will help generating new kenaf sterile resource and excellent cross combinations.