SSR Analysis of 32 Parents in Two-line Hybrid Rice

DNA of 24 male sterile lines and 8 restorer lines of two-line hybrid rice were amplified by using 20 SSR primer pairs of being screened.Two specific primer pairs were screened and selected to identify subspecies between Indica and Japonica.32 cultivars were clustered based on genetic distance(GD) by Unweighted Pair Group Method using Arithmetic Average(UPGMA).The results showed that 32 cultivars were divided into two groups(Indica and Japonica).In the indica group,sterile line Peiai64S was clustered into one group.7 sterile lines originated from AnnongS and restorer line Yuhong1 were clustered together.Of 5 Indica restorer lines,3 restorer lines were clustered together,2 restorer lines and sterile lines were clustered together.In the Japonica group,sterile line HN5S was clustered into one group.3 Japonica restorer lines were clustered together,which were distinguished from the Japonica sterile lines.Compared the genetic distance between 5 Indica restorer lines and Pei'ai64S,the genetic distance was remote correspondingly between 9311 and Pei'ai64S.It showed that there was relativity between the genetic distance and the heterosis of Liangyou Peijiu.