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Cloning of β-glucosidase genes from Tridchoderma viride into E.coli

Yin Liu

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Abstract

As total RNA of Trichoderma viride was the template.According to the DNA sequence of β- glucosidase genes(bgl I) from GenBank,specific primers were designed to amplificate fragments of bglⅠ by PT-PCR,then they were cloned into pMD18-T vector and transformed into E.coli JM109 to determinate sequence.Sequence analysis indicated that the β-glucosidase genes nucleotide similarity of DNA sequences among Trichoderma viride and others obtained from GenBank was 98.8%.

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What this paper is about

As total RNA of Trichoderma viride was the template.According to the DNA sequence of β- glucosidase genes(bgl I) from GenBank,specific primers were designed to amplificate fragments of bglⅠ by PT-PCR,then they were cloned into pMD18-T vector and transformed into E.coli JM109 to determinate sequence.Sequence analysis indicated that the β-glucosidase genes nucleotide similarity of DNA sequences among Trichoderma viride and others obtained from GenBank was 98.8%.

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Available abstract

As total RNA of Trichoderma viride was the template.According to the DNA sequence of β- glucosidase genes(bgl I) from GenBank,specific primers were designed to amplificate fragments of bglⅠ by PT-PCR,then they were cloned into pMD18-T vector and transformed into E.coli JM109 to determinate sequence.Sequence analysis indicated that the β-glucosidase genes nucleotide similarity of DNA sequences among Trichoderma viride and others obtained from GenBank was 98.8%.

Key concepts: GenBank, Trichoderma viride, Gene, Cloning (programming), Biology, Genetics, DNA, Sequence analysis

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