Cloning and Expression of β-Glucosidase Gene from Bacillus pumilus

Strain Bacillus pumilus AC-6 was separated from the soil, which can produce β-glucosidase. According to the β-glucosidase gene of strain SAFR-032, primers were designed to clone the β-glucosidase gene from AC-6. The sequence of β-glucosidase gene from AC-6 is 1437 bp and encode 478 amino acids. Compared with β-glucosidase gene reported in GenBank, the similarity of the gene sequence is 98%. We cloned β-glucosidase gene into the vector pGEX-4T-1 and transferred it into the competent E. coli DH5α and BL21（DE3）for expression.