Serotype,resistance,tdh and trh gene analysis on Vibrio parahaemolyticus isolates from food poisoning

Objective:To investigate the main epidemic strain type and correlative biological characteristics of the Vibrio parahaemolyticus isolates from food poisoning.Methods:The isolated strains of Vibrio parahaemolyticus were subjected to serotype using serum agglutination test,and adopting flat method to do the resistance detection.Meanwhile,adopting PCR to detect the tdh and trh.Results:In 56 strains,that were 54 strains from patients.O3:K6,O4:K8 and O1:K25 with tdh positive and trh negative were 49,2 and 2 strains.The two Vibrio parahaemolyticus strains isolated from food remains were different,one of 2007 with tdh negative and trh negative was O1:K56,one of 2008 with tdh negative and trh negative was not able to be serotyped.The 56 strains show large proportion or all resistance to Penicillins,show sensitive to Aminoglycoside,Quinolones and Sulfonamides.Conclusion:The Vibrio parahaemolyticus strains isolated from food-poisoning are mainly O3:K6 with tdh gene in Minghang district.