The isolation and morphological observation of umbilical cord blood mesenchymal stem cells from preterm infant

Objective:To explore a simple and pragmatic way of isolation and cultivation of mesenchymal stem cells(MSCs) derived from preterm infant umbilical cord blood.Methods:Umbilical cord blood were collected from preterm infant and full term deliveries.The umbilical cord blood mononuclear cells were isolated by density gradient centrifugation with lymphocyte separation medium.Using MesencultTM culture medium,the biological characteristics of MSCs derived from human umbilical cord blood were observed by detecting the cell surface marker by immunofluorescence method.Results:MesencultTM medium was the best medium,the achievement ratio of umbilical cord blood from preterm infant was 100%,higher than full term deliveries when the other conditions were the same.MSCs derived from human umbilical cord blood expressed CD29,CD44 and CD90,but not antigens of hematopoietic CD34.Conclusion:We have found the optimized method of culture of umbilical cord blood mesenchymal stem cells from preterm infant.