2007•Zhongnan Linye Keji Daxue xuebaoRequires access
Improvement on PAGE Silver Staining and Band Recovering
Peng Li-mei
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Abstract
To enhance the efficiency of silver staining and band recovering in Polyacrylamide Gel Electrophoresis(PAGE),both conventional silver staining and speedy silver staining were investigated,using the technique of ISSR-PCR markers with Magnolia grandiflora DNA.During band-recovering,conventional ethanol hepatin method and improved boiling method were employed and followed by re-amplification of PCR identification.The results indicate that the two methods produce similar clear bands and band patterns,but the procedure of conventional silver staining takes 2～3 hours while the speedy silver staining only requires 30 minutes which reduces the experimental time by 4～6 times.The improved boiling method is more convenient and time-saving than the conventional one.Speedy silver staining and improved boiling band-recovering can be employed in the future studies.