2009•Journal of Tangshan Teachers CollegeRequires access

Improvement of DNA Silver Staining Detection

Fan Yong-shan

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Abstract

Silver staining for DNA polyacrylamide gel electrophoresis(PAGE) is one important but difficult technique in Molecular Biology.The influencing factors,inculding the quantity of sample application,stationary time,and imaging time,were optimized for the PAGE/silver staining technique.The good experimental results showed that the improved method was feasible and available.

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What this paper is about

Silver staining for DNA polyacrylamide gel electrophoresis(PAGE) is one important but difficult technique in Molecular Biology.The influencing factors,inculding the quantity of sample application,stationary time,and imaging time,were optimized for the PAGE/silver staining technique.The good experimental results showed that the improved method was feasible and available.

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Available abstract

Silver staining for DNA polyacrylamide gel electrophoresis(PAGE) is one important but difficult technique in Molecular Biology.The influencing factors,inculding the quantity of sample application,stationary time,and imaging time,were optimized for the PAGE/silver staining technique.The good experimental results showed that the improved method was feasible and available.

Key concepts: Silver stain, Staining, DNA, Gel electrophoresis, Polyacrylamide gel electrophoresis, Molecular biology, Electrophoresis, Chromatography

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