Effection of Tonglian Decoction and its Formulae on Cell Proliferation and Cell Cycle of Cell Line Eca109 of Esophageal Carcinoma

Objective:To investigate effect of Tonglian decoction(T) on proliferation of esophageal carcinoma cell line Eca109 through determination of cell morphology and cell cycle,and to discuss inhibition mechanism of the prescription on esophageal carcinoma.Method: Human esophageal carcinoma cell line Eca109 cultured in vitro were treated with T and its separated formulae,including clearing-heat-and-detoxification formula(Q),activating-blood-and-promoting-Qi formula(H) and nourishing-Yin-and-blood formula(Z).Cell proliferation was assayed by MTT,cells morphology was observed with microscope and cell cycle was measured with Flow Cyto Meter(FCM).Result: Dose for 50 percent cells inhibition rates(IC50) of T,Q and H was 386,771,729 mg·L-1,respectively.T,Q and H inhibit cell proliferation predominantly,rate of G1 phase of cell cycle reached to 69.43%,60.84%,61.90%,respectively.Cells number in S phase treated with T,Q,and H had significant difference with controlled group(P0.05),and T had the best effect.But Z has little inhibition power,having no difference with controlled group.Conclusion: T can be used to regulate Eca109 carcinoma cell proliferation with inhibiting cells S phase.During whole process,3 separated formulae cooperate with each other to insure effect of T.