Effect of Galvano-acupuncture on the Expression of P-selectin and E-selectin of Cerebral Ischenic Region in Rats

Objective To study mechanisms of early treatment of cerebral ischemia-reperfusion(I/R) by galvano-acupuncture in resisting inflammation injury in rats.Methods Cerebral I/R model was established by occlusion of the middle cerebral artery for 1h and then removal of the occlusion for 3 h,12 h,24 h,and 48 h respectively.The expression of P-selectin and E-selectin was displayed by using imunohistochemical ABC in ischemic cerebral cortex and corpus striatum in rats.Results The expression of P-selectin and E-selectin was detected at capillary walls in postischemic cerbral cortex at 1 h ischemia-3 h reperfusion and peaked at 1h ischemia-24 h reperfusion and increased continuously during 48 h reperfusion.The expression of both P-selectin and E-selectin in ischemic cerebral cortex were down-regulated considerably by galvano-acupuncture treatment(P0.01).Conclusion The protective effect of acupuncture on cerebral neurons under acute infarction is possible through its downregulative action on the expression of P-selectin and E-selectin,which causes neutrophil infiltration that induced by adhesive molecules between endothelium cell and neutrophil.