Experimental studies of the expression of P-selectin and L-selectin in the rats with focal cerebral ischemia-reperfusion injury

Objective To evaluate the expression of P-selectin and L-selectin in the rats with focal cerebral ischemia-reperfusion and study the effect of P-selectin and L-selectin on cerebral ischemia-reperfusion injury.Methods 72 male SD rats were randomly divided into the sham operated group and cerebral ischemia 2 h with reperfusion 2,3,4,6,12,24,48 h group.The focal cerebral ischemia-reperfusion models of middle cerebral artery occlusion(MCAO) were made by inserting nylon surgical thread through the internal carotid artery(ICA) into the anterior cerebral artery(ACA).The expression of P-selectin and L-selectin were evaluated by immunohistochemical method at different reperfusion time points.Results P-selectin and L-selectin could not be detected in sham operated control group.P-selectin was detected as early as 3 h after reperfusion and peaked at 12 h and still could be detected at 48 h.Lselectin was detected as early as 2 h after reperfusion and peaked at 6h after reperfusion and still could be detected at 24 h.Conclusions The upregulated expression of P-selectin and L-selectin in the rats with focal cerebral ischemia-reperfusion suggests that P-selectin and L-selectin enhance leukocytes' adhesion to cerebral microvascular endothelial cells,and involve in the inflammatory injury of cerebral ischemia-reperfusion.