Expression of C-myc and HPV16/18 in cervical carcinoma and cervical intraepithelial neoplasia and their relationship.

Objective To investigate the expression of C-myc and HPV16/18 DNA in cervical carcinoma and cervical intraepithelial neoplasia (CIN) and their relationship with clinicopathology.Methods 10 cases of normal cervical epithelium,20 cases of cervical intraepithelial neoplasia (CIN) and 30 cases of cervical carcinoma were included.The expression of C-myc gene was evaluated by immunohistochemistry (Maxvision).The expression of HPV16/18 DNA was determined by hybridization in situ.Results (1)The positive expression rate of C-myc was 20%(2/10) in normal cervical epithelium,45%(9/20) in CIN and 66.7 %(20/30 ) in cervical carcinoma respectively.The positive expression rate of HPV16/18 DNA was 10%(1/10) in normal,50%(10/20) in CIN and 73.3 %(22/30 ) in cervical carcinoma respectively.Their difference were all significant(P0.0 5).(2)The positive expression rate of C-myc elevated with FIGO,histological grade and the lymph node metastasis.Their difference was significant(P0.0 5).(3)HPV16/18DNA was positively correlated with C-myc in cervical carcinoma.Conclusion (1) High-risk HPV was positively correlated with C-myc in cervical carcinoma,supportting that C-myc gene is a target gene of high-risk HPV.(2)HPV had the vital clinical significance of disease-screen and early diagnosis.(3)C-myc had important value to appraisal of the cervical carcinoma treatment result and estimate of the prognostic.