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Determination of thiamphenicol residues in honey by HPLC-UV

Ju Dawei, Mao-Sheng Chen, Hong Wu, Ruan XiangChun, YU Lin-sheng

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Abstract

A method for determination of thiamphenicol(TAP) residues in honey by using high performance liquid chromatography(HPLC) has been developed and validated.The sample was extracted from honey by alka-line ethyl acetate,concentrated by rotary evaporator,purified by C18 SPE(solid phase extraction) cartridge,and detected at a wavelength of 225 nm.The pre-treatment conditions were optimized in order to decrease the matrix interference.The detection limit for TAP was 30 μg?kg-1,less than 50 μg?kg-1 which was provided by Industry Standard of the People's Republic of China SN 0199-93.The average recoveries for TAP were between 71.3% and 90.9%.Coefficients of variation ranged from 2.0% to 3.4%.The method has good repeatability and high sensitivity,and can be applied for the determination of TAP residue in honey.

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What this paper is about

A method for determination of thiamphenicol(TAP) residues in honey by using high performance liquid chromatography(HPLC) has been developed and validated.The sample was extracted from honey by alka-line ethyl acetate,concentrated by rotary evaporator,purified by C18 SPE(solid phase extraction) cartridge,and detected at a wavelength of 225 nm.The pre-treatment conditions were optimized in order to decrease the matrix interference.The detection limit for TAP was 30 μg?kg-1,less than 50 μg?kg-1 which was provided by Industry Standard of the People's Republic of China SN 0199-93.The average recoveries for TAP were between 71.3% and 90.9%.Coefficients of variation ranged from 2.0% to 3.4%.The method has good repeatability and high sensitivity,and can be applied for the determination of TAP residue in honey.

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Available abstract

A method for determination of thiamphenicol(TAP) residues in honey by using high performance liquid chromatography(HPLC) has been developed and validated.The sample was extracted from honey by alka-line ethyl acetate,concentrated by rotary evaporator,purified by C18 SPE(solid phase extraction) cartridge,and detected at a wavelength of 225 nm.The pre-treatment conditions were optimized in order to decrease the matrix interference.The detection limit for TAP was 30 μg?kg-1,less than 50 μg?kg-1 which was provided by Industry Standard of the People's Republic of China SN 0199-93.The average recoveries for TAP were between 71.3% and 90.9%.Coefficients of variation ranged from 2.0% to 3.4%.The method has good repeatability and high sensitivity,and can be applied for the determination of TAP residue in honey.

Key concepts: Thiamphenicol, Chromatography, High-performance liquid chromatography, Detection limit, Residue (chemistry), Repeatability, Tap water, Solid phase extraction

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