2005•Progress in Veterinary MedicineRequires access
Establishment of a Multiplex-PCR for Detecting Actinobacillus pleuropneumoniae,Pasteurella multocida and Haemophilus parasuis
MI Feng-quan, Xiaoling Chen, Wang Cui-min, Yujun Zhao
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Abstract
A multiplex PCR detecting APP, PM and HPS was developed based on published single PCRs for these pathogens. The PCR reagents, annealing temperature and the PCR procedure were optimized. A 342 bp fragment of APP, a 457 bp of PM and a 821 bp of HPS can be simultaneously amplified in one reaction. The mPCR can detect minimum 100 cells of each species or 50 pg of APP and HPS DNA or 500pg of PM DNA in one reaction. The sensitivity of the mPCR is quite comparable with their reported uniplex PCRs. The assay is a potential tool for differentiation of the 3 pathogens and diagnosis of mixed infection by these bacteria.