2009•ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSISRequires access
Development and application of multiplex-PCR for identification of Actinbacillus pleuropneumoniae and Haemophilus parasuis.
Ying He, Ping Zhao, Yuefeng Chu, Pengcheng Gao, Jianjun Zhang, LU Zhong-xin
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Abstract
The multiplex PCR detecting App and PM were developed based on published single PCR for these pathogens.The PCR reagents,annealing temperature and the PCR procedure were optimized and the primers were screened.A 342 bp fragment of App,and a 457 bp of PM could be simultaneously amplified in one reaction.App was as low as 1.4×103 CFU or 9 pg DNA and PM was as low as 2.3×103 CFU or 16 pg DNA.For 23 suspected isolates,this multiplex-PCR method correctly identified 4 App and 2 PM.The result suggests the usefulness of the muhiplex PCR for routine identification of App and PM.