2003•Academic Journal of Second Military Medical UniversityRequires access

Development of transgenic mice harbouring hepatitis B virus core gene

Hong Liu, Yao Yucheng, Jin Hua He, Wenlin Li, Li Jianxiu, Xiaoping Su, Qinxian Zhang, Yiping Hu

Abstract

Objective: To establish the transgenic mice model harbouring hepatitis B virus (HBV) core gene (ayw type). Methods:The transgenic mice were generated by microinjecting core gene into fertilized eggs.The analysis of the HBV core gene integration in the transgenic mice was carried out by PCR, immunohistochemistry and H E staining. Results:Six founder transgenic mice were obtained. HBcAg was detected in hepatocellular nuclei and cytoplasm in one founder transgenic mouse.Hepatitis B virus core gene integrated in all 10 F 1 offsprings. HBcAg was detected in 4 of 10. Conclusion:The HBV core gene is integrated in the mice genome and can be expressed.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: HBcAg, Hepatitis B virus, Virology, Transgene, Biology, Genetically modified mouse, Gene, Molecular biology