Establishment of transgenic mice for hepatitis B virus core gene expression

Aim: To establish transgenic mice for hepatitis B virus core gene expression. Methods: The HBV core gene eukaryotic expression vector was constructed and the linerized DNA was microinjected into the male pronuclei of C57BL/6 mouse fertilized eggs. The eggs survived from microinjection were transplanted into oviducts of pseudopregnant recipients. The transgenic mice were identified by PCR. Results and Conclusion: The map of endonuclease digestion and sequencing of the eukaryotic expression vector was consistent with what in anticipation. The transgene positive mice accounted for 25%(6/24). The transgenic mice for hepatitis B virus core gene expression are established successfully.