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DGGE Analysis of Different Variable Region Fragments in 16S rRNA Gene of Pseudomonas from Chilled Meat

Zhou Guang-hong

Abstract

In this study,16S rRNA gene sequences of different Pseudomonas cloned from chilled meat were analyzed based on V3 and V6-V8 region fragments to explore the technical defects of denaturing gradient gel electrophoresis(DGGE).The results showed that different variable regions of 16S rRNA could lead to different DGGE results,and the(G + C) contents of variable region fragments were not related to the migrating distance in the DGGE.Therefore,the limitation of DGGE was due to the innate characteristics of bacterial 16S rRNA gene sequences to some extent.

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Key concepts: Temperature gradient gel electrophoresis, 16S ribosomal RNA, Pseudomonas, Biology, Gene, Food science, Bacteria, Microbiology