Protective Effects of Jieduhuayutang on Hepatic Injury in Mice Induced by D-galactosamine

ObjectiveTo investigate the protective effects of Jieduhuayutang(JDHYT) on liver injury in mice induced by D-galactosamine(D-GalN).Methods Sixty NIH mice were randomly divided into 5 groups: normal control group(group A),model group(group B),high dosage group of JDHYT(group C),small dosage group of JDHYT(group D) and Oleanolic Acid Tablets group(group E).Mice in group C,group D and group E were administered with drugs by gastric perfusion for 4 days once daily consecutively.One hour after the last administration of the drugs,excepts those in group A,mice in other groups were injected with D-GalN 800 mg/kg into its abdominal cavity.Blood samples for determining aminotransferase(ALT),superoxide dismutase(SOD) activity and malondiadehyde(MDA) concentration were collected at the 24h hour after D-GalN administration.Histopathological examination was also performed for liver tissue.ResultsSerum ALT and MDA in group C were obviously lower than that of group B(P0.01),SOD obviously higher than that of group B(P0.01),but there was no significant difference compared with group E(P0.05).Hepatic histopathological changes were alleviated in group C compared with group B(P(0.05)).ConclusionJDHYT has a good protective effects on liver injury in mice induced by D-GalN,its therapeutic mechanism is related to the antioxidation