Study of adhesion molecules β_2 integrin CD11c and CD18 expressions of peripheral blood leukocytes in patients with acute ischemic stroke

Objective To explore the expression and significance of adhesion molecules β2 integrin CD11c, CD18 in the polymorphonuclear neutrophils (PMN) and mononuclear leukocytes (MNL) of peripheral blood in patients with acute ischemic stroke (AIS).Methods Using indirect immunofluorescence and adopting a flow cytometry, the expressions of CD11c, CD18 in PMN and MNL in peripheral blood were measured within 72 h and 7 d after onset in 28 patients and 28 healthy controls.Results In AIS group, the expressions of CD11c, CD18 in PMN and MNL within 72 h ( 20.82±5.88, 218.25±89.00, 34.78±14.56 and 286.75±95.50, respectively) and at 7 d ( 18.60±5.52, 185.52±68.44, 31.97±14.47and 247.00±88.06, respectively) were significantly higher than those of normal control group ( 15.63±3.01, 150.76±41.20, 20.20±8.50 and 186.38±52.97, respectively)(all P0.01). Although CD11c and CD18 immunofluorescene intensity decreased at 7 d after onset of AIS, but there was not a significante difference in comparison with 72 h.Conclusion AIS may induce upregulation of β2 integrin CD11c and CD18 in activated PMN and MNL, and high level of CD11c and CD18 is correlated to the inflammatory response of AIS.