Coculture system containing marrow stromal cell layer supporting in vitro expansion of umbilical cord blood CD133+ cells

Objective To investigate the effects of expansion system containing bone marrow stromal cell layer on proliferation and differentiation of umbilical cord blood umbilical cord blood (UCB) haematopoietic stem/progenitor cells (HSPC) ex-vivo expansion and define the optimal means of expansion of UCB HSPC.Methods UCB CD133+ cells were cultured in expansion system containing bone marrow stromal cell layer established using bone marrow from patients with APL in CR and CML in CP. The number of NC, colonogeneic assay, immuno-phenotyping of the NC at every interval were monitored. Results The expanded cells could be collected easily in the culture containing marrow stromal cells. The number and the expansion folds of NC from stromal culture were significantly more than those from control. The number of CD34+, CD133+, CD34+CD38-cells and ratio of CD133+/CD34+ in stromal culture were remarkably higher than those in controls. The expansion of ~above subsets in NC from stromal culture was remarkably more than that from controls. Meanwhile, the plate efficiencies and expansion folds of the colonies of NC from stromal culture were also ~remarkably more than those from controls. Conclusion This expansion system could promote the ~expansion of UCB HSPC and also delay the differentiation of expanded cells, which could remain the primitive HPC in the expanded products and was helpful for engraftment of expanded cells.