Cloning Pathogenesis-related Gene Promoter of Arabidopsis and Construction of Plant Expression Vector

One pair of primer was designed based on the pathogenesis-related protein 1 of Arabidopsis thaliana L. Using PCR amplification, the promoter of PR-1 gene (910bp) was cloned from Arabidopsis to build inducible expression system of screening plant expression vector; by Sequence analysis, the 910 nucleotides promoter showed 99.7% homology with the reported sequence; the fragment was then instead of CaMV35S of the reporter gene encoding 3-glucuronidase (GUS); so a new plant expression vector named pBI-prp1 in which the reporter gene GUS is droved by promoter of pathogenesis related protein was constructed. It was transferred into Agrobacterium tumefaciens GV3101, then the transgenic Arabidopsis plants was obtained through Agrobacterium-mediated transformation which provided a reliable basis for the further study of host-pathogen interaction.