Online Column-switching with Reversed Phase High-performance Liquid Chromatography in Detection of Concentration and Pharmacokinetics of Buspirone in Plasma of Rats

Objective To detect concentration and pharmacokinetics of Buspirone in plasma of rats by using online column-switching with reversed-phase high-performance liquid chromatography( RP-HPLC) method. Methods The column of self-made restricted-access media was used as a pretreatment column,and a Luna C18column was used as analytical column. The mobile phase of pretreatment column was water-methanol( 95: 5,V /V),and the flow rate was1 ml /min; the mobile phase of analytical column was methanol-5 mmol /L ammonium formate( 75: 25,V /V),flow rate was 1 ml /min,the column-switching time was 3 min,and the column temperature was 25℃; the detection wavelength was 283 nm. Six Wistar rats were fasted for 12 h,and then were lavaged with 15 mg /kg doses of buspirone hydrochloride tablets,and 0. 5 ml blood through behind of the venous plexus of each rat's eyeball plexus was respectively collected at 0.08,0. 25,0. 5,0. 75,1,1. 5,2,4,6,8,12 and 24 h after the medication,and then the samples were centrifuged and injected with the supernatant. The area under the blood drug concentration-time curve,life of decay( t1 /2),maximum concentration( C max) and clearance( CLZ) during the medication were observed. Results There was a good linear correlation in 0. 2-4. 8 μg /ml concentration range of Buspirone in the rats' plasma( r = 0. 9993). The average recoveries of buspirone at 3 spiked levels( 0. 3,0. 6 and 2. 4 μg /ml) were 96. 03%,104. 67% and 105. 76%. The within-day and day to day precisions were less than 5%. The blood drug concentration-time AUC0→8,AUC0→∞,t1 /2,C max and CLZ during the medication were 3. 023 μg /( ml·h),4. 056 μg /( ml·h),3. 944 h,1. 38 μg /ml and 3. 712 L /( h·kg) respectively. Conclusion Pharmacokinetics of Buspirone in plasma of rats by using online column-switching with RP-HPLC method is simple,quick,accurate and suitable for the biological sample analysis.