Application of comparison method in internal quality control of hematology analyzer by using fresh blood

Objective To investigate the comparison method on internal control of hematology analyzer by using fresh blood.Methods Selecting the hematology analyzer with good functions and standardized operation as the reference analyzer,using healthy people's fresh blood with the values assigned by the reference analyzer to calibrate the compared hematology analyzer.Establishing the acceptable limits of relative deviation of WBC,RBC,HGB,HCT,PLT by comparative experiments among three months.Using fresh blood samples with low /medium/high levels from patients daily at least two levels,the results of compared analyzer is compared with reference analyzer's,calculate the relative deviation of WBC,RBC,HGB,HCT,PLT,respectively.Establishing the internal quality control charts in Laboratory Information System,with date as x-axis,relative deviation as y-axis and the acceptable relative deviation limits is set to be ±2s,the judgment whether it is out of control is based on the fresh blood comparison control rule worked out in laboratory.Results The x±s(%)of relative deviation of WBC,RBC,HGB,HCT,PLT with high,medium,low levels for compared analyzer are 0.75±2.964,1.19±2.488,1.43±2.439;-0.39±1.327,-0.26±1.297,-0.35±1.095;-0.43±1.393,-0.17±1.139,0.24±1.166;-0.43±1.362,-0.36±1.381,-0.57±1.299;-0.93±4.330,0.04±4.118,-0.41±4.149,respectively,in 2006.As the second instrument,the compared analyzer has been involved in College of American Pathologists Proficiency testing with satisfactory result and the bias(%)of WBC,RBC,HGB,HCT,PLT are within-0.5～5.1,-1.0～1.6,-1.7～1.4,-1.5～1.0,-4.5～7.4,respectively,in 2006.Conclusion This method has effectively,conveniently,economically performed quality control on compared hematology analyzer.