Effect of Ulinastatin injection on expression of high mobility group protein B1 in lung tissue of sepsis in rat

Objective To observe the lung tissue expression of high mobility group box 1 protein(HMGB1)in septic rats treating with Ulinastatin injection,and to investigate the action mechanisms of Ulinastatin in pulmonary function protect.Methods A total of 90 healthy male SD rats were randomly divided into three groups,and each group has 30 rats.Group A received sham operation and the others underwent cecal ligation and puncture(CLP)operation.All the rats were accepted intraperitoneal injection of normal saline(group A,B)or Ulinastatin(group C).Five rats in each group were sacrificed at 0,6,12,24,48 and 72 h after operation.Lung tissue express of HMGB1 in all rats were detected by reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemistry(IHC)methods,respectively.Results There have a very closely result while RT-PCR and IHC were used to detect HMGB1 expression in lung tissue.There have no significant difference in HMGB1 expression at all time points in Group A,but its get a top expression in Group B at 24 h and 48 h,and significantly higher than 0,6,12,72 h in Group B and the corresponding point in Group A and C(P0.01).The tendency in Group C and B is nearly,but the expression levels are significantly lower than Group B at 24 h and 48 h(P0.01),and the alveolar epithelial cellular edema are significantly lower than Group B in hematoxylin and eosin stain wave plates.Conclusions Ulinastatin is a reliable protection agent for septic induce acute lung injury,and its mechanism of action may be involved in the inhibition of HMGB1 expression.