Progress of Chromosome Walking by PCR Amplification Techniques

PCR-based chromosome walking is mainly applied to isolate flanking sequence of known sequence.It is convenient for gene cloning,obtaining regulatory elements and filling in gaps of whole genome sequencing.These methods can be classified into two sorts:dependent-ligation PCR and independent-ligation PCR.The technologies of PCR-based chromosome walking technologies in recent years and compares principles and procedures of these methods were outlined.The advantages and disadvantages of dependent-ligation PCR and independent-ligation PCR were summarized respectively,and their efficiency and usefulness were evaluated for investigator to select the appropriate method.