PCR Techniques for Chromosome Walking

Chromosome walking techniques are commonly used for cloning the known flanking sequence.This paper reviews the existing chromosome walking PCR techniques,such as inverse PCR,panhandle PCR,ligation mediated(LM) PCR,thermal asymmetric interlaced(TAIL) PCR and single oligonucleotide nested(SON) PCR,and also puts forward a new chromosome walking method.Using this method the objective products are amplified under common PCR proceeding with particular primer and random primer.Comparison experiments with corresponding random primer pairs are conducted to identify the non-objective products.This method may be a new and effective technique for chromosome walking.Fig 2,Ref 26