Effect of Bone Marrow Stromal Cells on Apoptosis of Rat Cirrhotic Fat-storing Cells

Objective To explore the effect on bone marrow stromal cells(BMSCs)on the apoptosis of rat cirrhotic fat-storing cells(CFSCs).Methods BMSCs,CFSCs and rat liver BRL cells were cultured by routine methods,and CFSCs and BMSCs were subjected to two-layer co-culture.Determine the concentrations of NGF,HGF and TGF-β1 in culture supernatant of BMSCs by ELISA,the expression of p75 in CFSCs after co-culture with BMSCs as well as in BRL cells by RT-PCR,and the effect of cytokines secreted by BMSCs,after blocking,on the apoptosis of CFSCs by TUNEL method.Results BMSCs secreted cytokines such as NGF,HGF and TGF-β1,and the secretion level increased with the increasing time for culture.No p75 was expressed in BRL cells.How-ever,p75 was expressed in CFSCs,and the expression level increased after co-culture of CFSCs and BMSCs.The proportion of apop-totic CFSCs induced by BMSCs after treatment with the blocking agent TAT-Pep5 of p75,the neutralizing antibody against HGF and the paralyser sp600125 of JNK decreased significantly.However,after blocking of TGF-β1,the proportion increased.Conclusion BMSCs promoted the apoptosis of CFSCs by secreting NGF and HGF.The promoting effect was JNK activity-dependent and increased after blocking of TGF-β1.