Dynamic expression of BDNF mRNA of cerebellar cortex after the infarction of rats middle cerebral artery supply area

【Objective】To observe the changes of BDNF mRNA in cerebellar cortex after rats middle cerebral artery occlusion.【Methods】Animal model was made with Longa’s Bolt line blocking method (MCAO) in middle cerebral artery of rats.Real-time quantitative PCR method was used to detect the expression of BDNF mRNA.【Results】The results of this study showed,after persistent middle cerebral artery occlusion,BDNF mRNA had the dynamic expression in the cerebellar cortex,and the expression of day 1,day 10 and day14 was significantly increased than the sham-operated group (P 0.05),while the expression of day 3,day 5 and day 7 was significantly decreased than the sham-operated group (P 0.05).【Conclusion】The mechanism of secondary damage of brain tissue away from infarction focal may be related to the neural inhibitory factor.