The inhibitory mechanism of endostatin on human umbilical vein endothelial cells(HUVEC)

Objective: To evaluate the inhibitory effect and the possible mechanism of endostatin on human umbilical vein endothelial cells(HUVEC).Methods: By means of MTT assay,the influences of endostatin with different concentrations(10～50μg/ml) and endostatin(30μg/ml) with different time(24～72h) on the HUVEC cells were studied.The changes of HUVEC cells' ultrastructures were examined by electron microscope.The influences of endostatin(30μg/ml) on the ectogenetic artificial blood vessel models were observed by photic microscope.Result: The growth of HUVEC cells was significantly inhibited by endostatin(20～50μg/ml)(P0.05,P0.01),with dose-time-dependence.Apoptosis was observed in the groups of HUVEC cells with endostatin by electron microscope.Endostatin could not only inhibit the formation of neonatal blood vessels but also destroy the formation of neonatal blood vessel nets.Conclusion: Endostatin can inhibit the proliferation of HUVEC cells with time-dose-dependence and it's mechanism maybe induces the apoptosis.It is pointed out that Endostatin can inhibit the proliferation of HUVEC cells,probably inducing the apoptosis,which can destory the neonatal blood vessels.Endostatin perhaps inhibits the growth and the transformation of human carcinoma according to this mechanism.