Determination of residual thiabendazole in the sugar orange by HPLC

A HPLC method for the determination of thiabendazole was described. Thiabendazole residue in the sugar orange was analyzed using a Hypersil ODS C18 column with UV-absorbance detector. The methanol was used as extracted solvent and the residual thiabendazole in the sugar orange was extracted with the ultrasonic extracted method. After liquid-liquid partition and thiabendazole was analyzed by HPLC,the HPLC conditions were as follows: methanol∶2%NH4Ac = 75∶25 (V/V) as mobile phase, flow rate being 1.0 mL/min, injection volume being 10 μL and detection wavelength at 300 nm. The results indicated that average recovery of thiabendazole in the sugar orange was 88.90%~95.37%, the lowest detection concentration was 0.02 μg/g, respectively which accorded to criterion of pesticide residues.