The growth of Nannochloropsis oculata cultured in different nutrient concentrations and the variations of nitrogen and phosphorus in culture tanks

The microalgae, Nannochloropsis oculata was cultured in tanks fertilized with 1, 2, 3 times of f/2 formulated nutrient respectively. Main components of f/2 formulated nutrient are NaNO 3 74.8mg, NaH 2PO 4 4.4mg, FeC 6H 5O 7·5H 2O 3.9mg. The results indicated: when fertilized with 2 times of f/2 formulated nutrient, N. oculata grow fastest. During the culture period, the concentrations of NH + 4-N, NO - 3-N, total nitrogen and PO 3- 4-P decreased. But the concentration of NO - 2-N decreased first and then increased. Under the high nutrient concentration, producing the unit biomass of N.oculata needs more nitrogen.