2021IOP Conference Series Earth and Environmental ScienceOpen access

Growth of Nannochloropsis oculata in shrimp cultivation waste at difference N:P ratios

Kharisma Nisa, Ahmad Shofy Mubarak, Laksmi Sulmartiwi

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Abstract

Abstract Shrimp culture waste contains high nutrients, namely N2, P2O5, K2O and has the potential to be used as organic fertilizer. Shrimp waste has a low N:P ratio so that it does not support microalgae growth, N:P needs to be adjusted for microalgae growth. The purpose of this study is determine the optimal N:P ratio in shrimp waste fertilizer which produced the highest growth ofNannochloropsis oculata. Method in this research is an experimental method. Completely Randomized Design (CRD) with 6 treatments and 3 replications. Treatment of shrimp waste fertilizer was used with different N: P ratios, N:P ratios of 10.5:1, 15.5:1, 20.5:1, and 25.5:1, namely : Walne fertilizer, N:P ratio 17:1 and Shrimp waste fertilizer with a ratio of N:P 5.9:1 is the control. Cultivation ofN.oculatauses an initial density of 105cells/mL, with light intensity ranging from 5.010-5.622 lux for 24 hours and lasts for seven days. Data analysis was processed used ANOVA then with DMRT. The results of this study had a significantly different effect on the population and growth rate ofN. oculata. The exponential phase highest population mean of 965x104cells/mL and the growth rate of 135x104cells/mL/day.

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Abstract Shrimp culture waste contains high nutrients, namely N2, P2O5, K2O and has the potential to be used as organic fertilizer. Shrimp waste has a low N:P ratio so that it does not support microalgae growth, N:P needs to be adjusted for microalgae growth. The purpose of this study is determine the optimal N:P ratio in shrimp waste fertilizer which produced the highest growth ofNannochloropsis oculata. Method in this research is an experimental method. Completely Randomized Design (CRD) with 6 treatments and 3 replications. Treatment of shrimp waste fertilizer was used with different N: P ratios, N:P ratios of 10.5:1, 15.5:1, 20.5:1, and 25.5:1, namely : Walne fertilizer, N:P ratio 17:1 and Shrimp waste fertilizer with a ratio of N:P 5.9:1 is the control. Cultivation ofN.oculatauses an initial density of 105cells/mL, with light intensity ranging from 5.010-5.622 lux for 24 hours and lasts for seven days. Data analysis was processed used ANOVA then with DMRT. The results of this study had a significantly different effect on the population and growth rate ofN. oculata. The exponential phase highest population mean of 965x104cells/mL and the growth rate of 135x104cells/mL/day.

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Available abstract

Abstract Shrimp culture waste contains high nutrients, namely N2, P2O5, K2O and has the potential to be used as organic fertilizer. Shrimp waste has a low N:P ratio so that it does not support microalgae growth, N:P needs to be adjusted for microalgae growth. The purpose of this study is determine the optimal N:P ratio in shrimp waste fertilizer which produced the highest growth ofNannochloropsis oculata. Method in this research is an experimental method. Completely Randomized Design (CRD) with 6 treatments and 3 replications. Treatment of shrimp waste fertilizer was used with different N: P ratios, N:P ratios of 10.5:1, 15.5:1, 20.5:1, and 25.5:1, namely : Walne fertilizer, N:P ratio 17:1 and Shrimp waste fertilizer with a ratio of N:P 5.9:1 is the control. Cultivation ofN.oculatauses an initial density of 105cells/mL, with light intensity ranging from 5.010-5.622 lux for 24 hours and lasts for seven days. Data analysis was processed used ANOVA then with DMRT. The results of this study had a significantly different effect on the population and growth rate ofN. oculata. The exponential phase highest population mean of 965x104cells/mL and the growth rate of 135x104cells/mL/day.

Key concepts: Shrimp, Fertilizer, Animal science, Nutrient, Population, Completely randomized design, Biology, Chemistry

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