The Effect of Edaravone on Expression of NGF and BDNF in Ischemia Stroke Tissue of Rat Cerebrum

Objective To study the effects of Edaravone on the protein and mRNA expression of brain derived neurotrophic factor(BDNF) and nerve growth factor(NGF) in the permanent focal cerebral ischemia tissues of rats.Methods Healthy male Wister rats were taken for this study project.According to the procedure of Zea-Longa,the rat model with permanent cerebral ischemia was established by rat middle cerebral artery obstructed(MCAO) with a nylon thread.Operated rats were randomly divided into model control and edaravone treated groups(n=20 for each).By intraperitoneal injection,model control group rats were given physiological saline 3mg/kg once daily for 3days,and also edaravone treated group rats were given edaravone 3mg/kg once daily for 3days.5 rats in each group were taken to tetrazolium chloride(TTC) staining for macroscopic observation of cerebral infarction area,the rest samples were processed by immunohistochemistry to evaluate effects of edaravone on BDNF and NGF expression,hybridization in situ to evaluate effects of edaravone on BDNF mRNA and NGF mRNA expression.SPSS12.0 for statistical analysis,it was p 0.05 as having statistical significance.Results Comparing to model control group,edaravone treated group did not have significantly pathological difference,but the grade of behavior and infarction area were apparently reduced(p 0.05).In edaravone group,there was a significant expression up-regulation to BDNF,NGF,BDNF mRNA and NGF mRNA in the peripheral around infarction and cornu ammonis or hippocampus area(p 0.05).However in the infarction area,the expressions of BDNF,NGF,BDNF mRNA and NGF mRNA had no significantly statistical difference(p 0.05).Conclusion Comparing to control group,edaravone can significantly up-regulate the expressions of BDNF and NGF in genetic transcription level,and protect from the ischemia injury.