Influence of EMP on the C-FOS expression in hippocampus

ObjectiveTo explore the possible injury mechani sm in brain of rat irradiated by EMP through observing the changes in expression of CFOS in hippocampus and dentate gyrus.MethodsThe group A, group B and group C were irradiated by high field intensity(6104,2104 a nd 6~{!A~}103 V/m respectively)electromagnetic puls es(EMP)for 5 times within 2 minutes respectively and no treatment was given to g roup D(control group).The rats were sacrificed at 1, 6, 12,24 and 48 h,sep arately. Immunohistochemical method was used to investigate the expression of C FOS and image analysis for CFOS p ositive cells was performed by CMTAS~{*2r~} image analysis system at a magnificatio n of 400. Results The rats of irradiated groups had high expres sion of CFOS in neurons of hippocampus and dentate gyrus at 6-12 h, and the nu mber of CFOS positive cells was significantly increased as compared with contr ol group ~{*)~}(P0.05)~{**~}.Conclusions The EMP could cause significant high CFOS expression in hippocampus and gyrus,which may pla y an important role in the injury of rat brain by EMP.