All-trans-retinoic acid induced differentiation and apoptosis of human lung epithelial carcinoma cell line A549

Objective:To investigate the potential roles and mechanism of all-trans-retinoic acid(ATRA) in human lung epithelial carcinoma cell line A549 in vitro.Methods: Routine culturated A549 cell of logarithmic period of growth was devided into 5 groups: drug concentration of 1×10~(-5)mol/L,1×10~(-6)mol/L,1×10~(-7)mol/L,control group and blank control group.MTT was detected in all groups in 48 hours after adding ATRA,FCM were detected in 1×10~(-5)mol/L,1×10~(-6)mol/L concentraction groups and control group on the 6 day and observe the cells of 1×10~(-5)mol/L,1×10~(-6)mol/L concentration groups by electronic microscopy at the meantime.Results: ATRA of different concentration had obvious inhibition effect on cell proliferation and had statistic significance compared with negative control group(P0.01).Proliferation inhibition had dose-dependent manner(r=0.968).The cell were blocked in G0/G1 stage.Apoptotic cells were found only in 10 mol/L ATRA group by electronic microscopy.Conclusion: ATRA could both induce differentiation and apoptosis of A549 cells.This observation may provide evidence for ATRA in chemoprevention of lung cancer.