Relation between effect of all-trans retinoic on expression of NF-kB and NO in HL-60 cells and cell cycle

To explore the mechanism of differentiation of HL-60 cells induced by all-trans retinoic acid. HL-60 cells were cultured in RPMI 1 640 medium with NS, ATRA, ATRA+SNP and ATRA+L-NMMA, respectively. After treating the cells for 48 h, the expression of NF-kB, cell cycle were examined by FCM,and the NO levels were determined by nitric acid reduction method. The expression of NF-kB in treating groups was significance decrease than in control group (P0.05), but that in ATRA+SNP group was the lowest. The NO levels in ATRA and ATRA+SNP groups were significantly high than those of contral groups(P0.05), but the NO level of ATRA+L-NMMA group was no significantly high (P0.05). Ratio of G1 cycle cells in ATRA+L-NMMA group was significance decrease than in ATRA group (P0.05), then Ratio of G1 cycle cells was significance high than ATRA group (P0.05). [Conclusions] All-trans retinoic can induce differentiation of HL-60 by expression of down-regulate NF-kB and promoting NO levels.