Effect of cyanin on VEGF in Müller cells under high glucose and high insulin condition

Objective To evaluate expression of vascular endothelial growth factor(VEGF)in cultured rat retinal Muller cells under high glucose and high insulin condition,and effect of cyanin on VEGF expression.Methods Muller cells from rat retinas were collected and cultured in vitro.Muller cells were treated with 50 mmol·L-1 glucose and different concentrations of insulin for 24 hours.There were three groups:high insulin group with 3 kU·L-1 insulin,low insulin group with 3 U·L-1 insulin,control group with no insulin.Different concentrations of cyanin(121.1 μmol·L-1,22.8 μmol·L-1 and 0 μmol·L-1)were added to the medium in each group.At 48 hours after incubation,enzyme linked immunosorbent assay was used to detect VEGF in the supernatant fluid.Immunocytochemistry was applied to quantify VEGF expression in Muller cells.Results After high insulin treatment,the concentrations of VEGF in the supernatant fluid and Muller cells increased in all groups(both P0.05),but low and high concentrations of cyanin could decrease the expression of VEGF in supernatant fluid and Muller cells,and had significant differences with that without cyania(both P0.05),either no difference was found between two concentration of cyanin(P0.05).Conclusion Insulin with high concentration can up-regulate expression of VEGF in Muller cells,which can be inhibited by cyanin.