Optimization of Culture Conditions of Se-enriched Ganoderma lucidum

[Objective] To find the optimal culture conditions of Se-enriched Ganoderma lucidum mycelium.[Methods] The effects of carbon source,nitrogen source and fermentation conditions on the dry weight,total selenium and organic selenium content of Se-enriched G.lucidum mycelium were studied by using single factor test and orthogonal test.[Result] Under conditions of 0.01% sodium selenite content,glucose content 4%,potato extracting 20%,peptone content 1%,KH2PO4 0.3%,MgSO4 0.15%,pH=7,inoculation amount 10%,culture temperature 28 ℃,and shaking speed 180 r/min,the dry weight of Se-enriched G.lucidum mycelium was 1.528 g/100 ml,and the organic selenium content was 4 115.36 μg/g.[Conclusion] The optimal culture conditions of Se-enriched G.lucidum were determined,which could provide basis for development and utilization of Se-enriched G.lucidum.