Comparative Research on Proliferation in Vitro of Dendritic Cells from Different Sources of Human Peripheral Blood

【Objective】To compare the peripheral blood and cord blood dendritic cells(DC) by proliferation in vitro in order to get a more accessible source of DC.【Methods】Leukocytes were isolated with Ficoll diatrizoate gradients from normal peripheral blood and cord blood.Cells were washed and then incubated with complete culture system with GM-CSF 、TNF-α and IL-4.Detecting phenotype(CD1α、CD83、CD80、HLA-DR) and morphology observation on the 4th、8th、10th day,and comparing their ability to activate allo-MLR were made.【Results】Cellular number of DC from cord blood increased over peripheral blood(cord blood 2.05±0.05,peripheral blood 0.45±0.01,P0.05),there was significant difference between the two groups.No differences of cell morphology and phenotype were found from the peripheral blood and cord blood DC(67.2±3.6)% vs(69.3±6.8)% for CD1α,(73.7±5.5)% vs(75.6±4.9)% for CD83,at 10th day(P0.05).The proliferation ability of DCs from two groups in allogenic mixed lymphocyte reaction(AMLR) was of no significant difference(P0.05).【Conclusion】The mature DC is successfully induced from human cord blood and peripheral blood.In particular the cellular number of DCs from cord blood significantly increases over peripheral blood.