2006•Europe PMC (PubMed Central)Requires access
The direct cytotoxic effect of dendritic cells from human peripheral blood and cord blood upon the cervical carcinoma cells lines
Huali Wang, Beihua Kong, Jianhua Zhang, Ang Cai
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Abstract
The biological characeristics and their direct effect of dendritic cells(DC) from human peripheral blood and cord blood were investigated,in which DC were induced in vitro by cultivation of the monuclear cells from human peripheral blood and cord blood in the presence of cytokines,such as rhGM-CSF,rhIL-4 and rhTNF-α,and the related phenotype of DC was determined by flow cytometery(FCM).Meanwhile,the direct cytotoxis effect of DC from human peripheral blood and cord blood upon the carcinoma cell lines HeLa and CaSki cells was detected by MTT assay.It was found that the induced cells from the human peripheral blood and cord blood showed typical morphology of dendritic cell after 6 days cultivation.The FCM analysis demonstrated the high expression of HLA-DR((99.29)% and 98.14%),CD86(99.45% and 90.92%) and CD209((87.44)% and 83.14%) on these cells,respectively,while the expression of CD14 was rather low(0% and 3.2% respectively).As demonstrated by the MTT assay the cytotoxic effect of the DC derived from human peripheral blood and cord blood upon the carcinoma cell lines HeLa cells as well as CaSki cells with similar biological characteristics was obviously found.This experimental result provides a new idea in the immunothrapy for tumors.