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2009•Chinese Journal of Clinical Laboratory ScienceRequires access

Study on loss of OprD_2 in imipenem-resistant Pseudomonas aeruginosa

Tao Li

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Abstract

Objective To investigate the loss of outer membrane protein D2 (OprD2) gene in imipenem-resistant Pseudomonas aeruginosa.Methods The minimal inhibitory concentrations (MIC) of 10 antimicrobial agents against 80 strains of imipenem-resistant clinical isolates were determined by agar dilution method.PCR was used to detect OprD2 gene.Results PCR results showed that 44 of the 80 isolates of imipenem-resistant P.aeruginosa were positive for OprD2 gene.There was significant difference (P0.05) about the resistance ratio of CAZ and ATM between OprD2 gene positive and negative groups.Conclusions The loss of OprD2 gene is one of the main mechanisms for the imipenem resistance of P.aeruginosa and is related to the resistance of CAZ and ATM.

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What this paper is about

Objective To investigate the loss of outer membrane protein D2 (OprD2) gene in imipenem-resistant Pseudomonas aeruginosa.Methods The minimal inhibitory concentrations (MIC) of 10 antimicrobial agents against 80 strains of imipenem-resistant clinical isolates were determined by agar dilution method.PCR was used to detect OprD2 gene.Results PCR results showed that 44 of the 80 isolates of imipenem-resistant P.aeruginosa were positive for OprD2 gene.There was significant difference (P0.05) about the resistance ratio of CAZ and ATM between OprD2 gene positive and negative groups.Conclusions The loss of OprD2 gene is one of the main mechanisms for the imipenem resistance of P.aeruginosa and is related to the resistance of CAZ and ATM.

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Available abstract

Objective To investigate the loss of outer membrane protein D2 (OprD2) gene in imipenem-resistant Pseudomonas aeruginosa.Methods The minimal inhibitory concentrations (MIC) of 10 antimicrobial agents against 80 strains of imipenem-resistant clinical isolates were determined by agar dilution method.PCR was used to detect OprD2 gene.Results PCR results showed that 44 of the 80 isolates of imipenem-resistant P.aeruginosa were positive for OprD2 gene.There was significant difference (P0.05) about the resistance ratio of CAZ and ATM between OprD2 gene positive and negative groups.Conclusions The loss of OprD2 gene is one of the main mechanisms for the imipenem resistance of P.aeruginosa and is related to the resistance of CAZ and ATM.

Key concepts: Imipenem, Pseudomonas aeruginosa, Microbiology, Agar Dilution Method, Agar dilution, Minimum inhibitory concentration, Biology, Antimicrobial

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