Expression of Osteopontin and Osteocalcin mRNA in Ectomesenchymal Stem Cells under Induction of EMPs & EMD in Vitro.

Objective: Ectomesenchymal stem cells (EMSCs) are considered to be able to differentiate toward a cementoblast/osteoblast phenotype. This study is to investigate Osteopontin and Osteocalcin mRNA expression of Rat ectomesenchymal stem cells under inducing condition of EMPs EMD in vitro. Methods: Cultured EMSCs were exposed to the EMPs- or EMD- or (EMPs+EMD)- conditioned culture media. Osteoblast MC3T3-E1 served as positive control (P) and non-induced EMPs as negative control (N). Then Osteopontin and Osteocalcin mRNA expression were measured by RT-PCR technique. Result: 1) There was expression of Osteopontin mRNA in group EMPs, group EMD and group (EMPs+EMD) after induction for 3 weeks ; 2) There was expression of Osteocalcin mRNA only observed in group (EMPs+EMD). Conclusion: The cultured Rat EMSCs kept non-osteogenic differentiated state by itself; but may have osteogenic or cementogenic potential testified by expression of Osteopontin and Osteocalcin mRNA when induced by EMPs.