Culture and characteristic identification of bone mesenchymal stem cells in ovariectomy rats

PURPOSE:To investigate the cultivation method of BMSCs in ovariectomy rats,and observe the biological characteristics.METHODS: The animal model of osteoporosis was established by performing ovariectomy on SD rats.Bone marrow mesenchymal stem cells(BMSCs) in humerus,femur,tibia of OVX rats and Sham rats were isolated from the rats of ovariectomized(OVX) group and sham group respectively by means of adherence.The proliferation was tested by MTT,and the expression of antigens on cell surface was detected by flow cytometry.BMSCs in humerus was subject to osteogenic induction,and adipogenic induction;then,the difference in differentiation between the BMSCs(OVX) and BMSCs(Sham) was investigated.The data was statistically analyzed with SPSS16.0 software package.RESULTS: BMSCs in femur and tibia were not successfully cultured;BMSCs in humerus were cultured.Compared with BMSCs(Sham),the ALP activity and OCN secretion in BMSCs(OVX) was low.The number of lipid droplets in BMSCs(OVX) was more than that in BMSCs(Sham) after adipogenic induction.CONCLUSION: BMSCs(OVX) can be successfully cultured by selecting humerus marrow and increasing cell culture density;BMSCs(OVX) were characterized by low proliferation rate,weak osteogenesis ability and greater adipogenic ability.