Basic Analyses of Grass Carp by Means of RAPD
Zhang De
Abstract
Zhang De
Abstract
decamer primers were used for RAPD (Random amplified polymorphic DNA) analyses in grass carp (Ctenopharyngodon idells) in this study All primers produced bands ranging from 0 1kb to 3 0kb Total 117 distinct and highly repeated fragments were obtained by using 20 primers Among primers studied, only 6 primers (33 3% of total 20 primers) produced polymorphic bands among different individuals of grass carp The study provided date for analysis of genetic diversity of grass carp by RAPD markers
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decamer primers were used for RAPD (Random amplified polymorphic DNA) analyses in grass carp (Ctenopharyngodon idells) in this study All primers produced bands ranging from 0 1kb to 3 0kb Total 117 distinct and highly repeated fragments were obtained by using 20 primers Among primers studied, only 6 primers (33 3% of total 20 primers) produced polymorphic bands among different individuals of grass carp The study provided date for analysis of genetic diversity of grass carp by RAPD markers
Key concepts: RAPD, Grass carp, Biology, Genetic diversity, Genetics, DNA, Fish <Actinopterygii>, Fishery