Screening of Polymorphic Primers and Polymorphic Locus for Grass Carp Genomic RAPD Analysis

In order to establish a molecular marker system for grass carp genomic DNA polymorphism analysis, 31 polymorphic primers with which polymorphic products could be produced have been screened from 180 RAPD(Random amplified polymorphism DNA) primers. With these 31 polymorphic primers, total 327 distinct and highly repeatable fragments have been obtained by method of PCR. The length of the amplified fragments ranges from 400 to 2 000 base pairs. The number of fragments produced by a single primer varies from 5 to 14. Total 93 polymorphic loci have been detected with the 31 polymorphic primers and the frequency of the alleles on each polymorphic locus has been analyzed by statistical method. Both the 93 polymorphic loci and the 31 polymorphic primers might provide a reliable molecular marker system for grass carp genomic DNA polymorphism analysis.